Journal: The Journal of Neuroscience
Article Title: Behavioral Modulation and Molecular Definition of Wide-Field Vertical Cells in the Mouse Superior Colliculus
doi: 10.1523/JNEUROSCI.1816-24.2025
Figure Lengend Snippet: Ntsr1+ SC neurons input and output characterization. A , Photomicrographs of Cre-dependent pseudotyped rabies tracing of Ntsr1+ neurons. Starter cells in the SC are highlighted in magenta. Cell bodies were identified in the visual cortex, retina, pretectum, parabigeminal nucleus (PBG), and ventral lateral geniculate nucleus (vLGN). Three mice. B , Photomicrograph of the injection site and projection pattern of Ntsr1+ neurons in the midbrain after injection of an AAV carrying Cre-dependent tdTomato (Ntsr1+ neurons) together with an AAV carrying CAG-driven GFP (all SC neurons). The top row shows the injection site, and the bottom row shows projections in the thalamus. Scale bar, 100 µm, three mice. C , Photomicrograph of the injection site and projection pattern of Ntsr1+ neurons in the midbrain after injection of an AAV carrying Cre-dependent tdTomato only. D , Immunolabeling of the projections of neurons labeled in C for VGluT2 and synaptophysin. E , F , Same as C and D for the thalamus, showing the pulvinar and corresponding inputs from the Ntsr1+ SC neurons. Scale bars: C and D , 500 µm; D and F , 10 µm. G , Absolute quantification of putative synapses originating from Ntsr1+ neurons in the SC and their immunolabeling in the pulvinar and PBG. Wilcoxon signed rank test, p < 0.05, indicated with a star. H , Proportion of putative synapses labeled in the PBG and pulvinar. Four mice.
Article Snippet: The viral titer was >10 12 GC/ml for AAV1.Flx.Split.TVA.B19G (catalog #52473, Addgene, RRID:Addgene_52473-AAV1) and >10 8 GC/ml for pseudotyped rabies virus (EnvA-coated G-deleted rabies-mCherry, Salk Institute, catalog #32636, Addgene, RRID:Addgene_32636).
Techniques: Injection, Immunolabeling, Labeling, Quantitative Proteomics
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